MUC1 Tandem Repeat Structural Studies Using Nuclear Magnetic Resonance Spectroscopy.

Abstract

In this project we employed the Solid Phase Peptide Synthesis Method to synthesize mucin peptides. The methodology for the synthesis of linear and cyclic mucin peptides are presented here. The amino acid sequence of the mucin peptides were derived from the tandem repeat of the mucin-1 (MUC-1) protein. 2D 1H NMR is employed to study the structures of these peptides. Subsequent studies will focus on the binding properties of mucin peptides to monoclonal antibody expressed against tandem repeat of the MUC-1 protein.