Heterologous Expression of MBP1 from C. ablicans in S. cerevisiae : a Nontraditional approach

Abstract

Cases of hospital-acquired fungal infections, such as oropharyngeal candidiasis. Recent studies of ICU fungal infections consistently find C. albicans to be the predominant yeast species isolated. We have found MBP1 to be toxic to bacteria, therefore traditional methods for obtaining the expression construct will not work. A new protocol is being used where S. cerevisiae is transformed directly with the vector construct and cultured on selective media. Vector is then isolated and PCR amplified to confirm presence of MBP1. Results of this work will contribute to our understanding of yeast morphogenesis and provide a protocol for studying genes that are toxic to bacteria.