Attempts to analyze the RNA-binding specificity of the C. elegans RNP-8 protein
| dc.contributor.advisor | Kim, Kyung Won (Mentor) | |
| dc.contributor.advisor | Kimble, Judith (Mentor) | |
| dc.contributor.author | Wilson, Tracy | |
| dc.date.accessioned | 2010-07-21T17:41:37Z | |
| dc.date.available | 2010-07-21T17:41:37Z | |
| dc.date.issued | 2010 | |
| dc.description | 29 p. | en |
| dc.description.abstract | Cytoplasmic polyadenylation is a key mechanism of gene regulation in all eukaryotes. The C. elegans GLD-2 protein provides the catalytic activity for a cytoplasmic poly(A) polymerase, but GLD-2 must partner with another protein that provides RNA-binding activity to produce a fully functional enzyme. One such GLD-2 binding partner is RNP-8, which contains an RNA recognition motif. Here, we explored the RNA-binding specificity of RNP-8. Using the yeast-three hybrid system, we identified 34 unique RNA sequences that were bound by RNP-8, but we could not identify a consensus sequence among the 34 sequences. Therefore, RNP-I protein may lack sequence specificity when binding to RNA. | en |
| dc.identifier.uri | http://digital.library.wisc.edu/1793/46048 | |
| dc.language.iso | en_US | en |
| dc.rights | The author grants to the University of Wisconsin-Madison the permission to reproduce and to distribute publicly paper and electronic copies of this thesis document in whole or in part in any medium now known or hereafter created. | en |
| dc.subject | Biochemistry | en |
| dc.subject | Biology | en |
| dc.title | Attempts to analyze the RNA-binding specificity of the C. elegans RNP-8 protein | en |
| dc.type | Thesis | en |
| thesis.degree.level | BS | en |