Protein Expression in Breast Tissue Challenged With Environmental Estrogens

Abstract

Estrogen (E2) has many beneficial effects but paradoxically estrogen exposure increases cancer risk in the breast and in the uterus. Bisphenol A (BPA) and methylparaben (MP) are environmental estrogens that are known to influence the expression of estrogen sensitive genes. To further elucidate the mechanism of action of environmental estrogens in breast cancer cells, we treated T47D and MCF-7 cells with MP and BPA, respectively, and measured the expression of proteins in the E2 signaling pathway utilizing Coomassie blue staining and Western Blotting.