Characterization of an Eleven Residue MUC-1 Peptide Structure in Solution by 2D NMR Sprectroscopy

Abstract

MUC-1 peptides are peptides based on the amino acid sequence of the tandem repeat domain of mucin expressed by cancer cells, which immunity in a host could be induced against. MUC-1 peptides have been used as antigenic agents in the development of cancer vaccine. The purpose of this research is to better understand the specific conformation of a specific MUC-1 peptide. To address the question, we have synthesized an 11-mer peptide with the sequence GVTSAPDTRPA that spans the main portion of the tandem repeat domain of mucin that is known to bind to MUC-1 monoclonal antibody.