Progress Towards Identifying LRB1 LRB2 Enhancer Mutations in the Plant Arabidopsis Thaliana by Next-Generation Genome Sequencing and Mapping

Abstract

We mutagenized lrb1 lrb2 plants, and then looked for individuals where the red light hypersensitivity was suppressed or enhanced. These plants likely carry a mutation in a gene involved, like LRB1 and 2, in light responses. We successfully identified numerous suppressor and enhancer mutants, two of which are discussed here. Our current focus is to map the mutations in these lines, with the hope of identifying new genes not previously implicated in light responses. To identify the mutations, we are using a whole-genome sequencing and next-generation sequencing strategy, described here.